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Skills and Competencies

In vitro & in vivo

  • Cell and tissue culture

    • mammalian cell lines & primary cells 

    • organoid & spheroid models

    • sophisticated co-culture models

    • cell based assays

  • Molecular biology

    • RT-qPCR, PCR, electrophoresis, Western blot

    • bacterial transformation & traditional cloning

    • transfection & transduction

    • DNA, RNA, and protein isolation

  • Genome editing

    • RNAi mediated knock-down

    • CRISPR/Cas9 knock-out & knock-in

  • Immunofluorescence and confocal microscopy (Nikon SoRa)

  • Spectral flow cytometry (Cytek Aurora)

  • Viral gene delivery

    • 3rd generation lentiviruses​

    • Ad5 adenoviruses

    • y-retroviruses

  • Live cell in vitro and in vivo imaging

    • mainly bioluminescence reporters ​

    • some experience with fluorescent reporters 

  • Mouse colony planning and genotyping

  • Tail vein and IP injection

Computational & omics

  • R programming language

  • Bulk, single cell, and timeseries RNA-Seq analyses

    • DESeq2, pheatmap, PCAtools

    • Seurat​, SingleCellExperiment, scran, dittoSeq

    • JTK_CYCLE, MetaCycle, dryR, comparerythms

  • Circadian rhythm analyses

    • chronoStar, pyBOAT​

  • Cell tracking

    • p53-Cinema​

  • Spectral flow data analysis (R, FlowJo)

  • Immunofluorescence image analysis (ImageJ)

  • Statistical analysis (Prism, R)

Soft skills

  • Strategic and analytical thinking, problem-solving

  • Teamwork and collaboration

  • Supervision and mentoring

  • Time and project management

  • Organization and record keeping

  • Scientific writing and presentation

Languages

  • German - native 

  • English - fluent

  • French

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